Interaction Between HOX-C11 and the Steroid Receptor Co-activator-1in Hormone Resistant Breast Cancer

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Associations between steroid receptor coactivator proteins (SRC) and the development of resistance to endocrine treatment have been described in human breast cancer. It has previously been shown that SRC-3, but not SRC-1, can be recruited to tamoxifen/ligand bound ER in the presence of the growth factor receptor HER-2. This mechanism may be implicated in endocrine dependent resistance leading to recurrance. Interaction of SRC-1 with a non-ER transcription factors may be implicated in the developement of endocrine independent resistance in recurrent breast cancer.In this study it was hypothesized that SRC-1 may interact with the transcription factor HOX-C11 in tamoxifen insensitive breast cancer cells (LY2 cells) compared to tamoxifen sensitive breast cancer cells (MCF-7 cells).Experimental design: SRC-1 interacting proteins were isolated by Co-immunoprecipitation, separated by SDS-PAGE, using Silverstaining and Western Blot to detect proteins. Immunohistochemistry was used to detect if HOX-C11 and SRC-1 were expressed in breast cancer tissue.Results: Both co-activator protein SRC-1 and transcription factor HOX-C11 were expressed in breast cancer tissue. The interaction between SRC-1 and HOX-C11 in LY2 cells was confirmed.Conclusions: Alternative co-activator-transcription factor interactions may be a mechanism for e...